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Tag: RT-PCR

UncategorizedPosted onMay 14, 2020

Performance of the rapid Nucleic Acid Amplification by Abbott ID NOW COVID-19 in nasopharyngeal swabs transported in viral media and dry nasal swabs, in a New York City academic institution

The recent emergence of the SARS-CoV-2 pandemic has posed formidable challenges for clinical laboratories seeking reliable laboratory diagnostic confirmation. The swift advance of the crisis…

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UncategorizedPosted onMay 7, 2020May 7, 2020

Interpreting Diagnostic Tests for SARS-CoV-2

The pandemic of coronavirus disease 2019 (COVID-19) continues to affect much of the world. Knowledge of diagnostic tests for severe acute respiratory syndrome coronavirus 2…

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UncategorizedPosted onMay 3, 2020

Presymptomatic SARS-CoV-2 Infections and Transmission in a Skilled Nursing Facility

Twenty-three days after the first positive test result in a resident at this skilled nursing facility, 57 of 89 residents (64%) tested positive for SARS-CoV-2.…

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UncategorizedPosted onMay 2, 2020

Shedding of infectious SARS-CoV-2 in symptomatic neonates, children and adolescents

Children are underrepresented in COVID-19 case numbers, with most pediatric cases exhibiting limited severity, and do not seem to be major drivers of transmission, unlike…

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UncategorizedPosted onApril 21, 2020

Estimating false-negative detection rate of SARS-CoV-2 by RT-PCR

Reverse transcription-polymerase chain reaction (RT-PCR) assays are used to test patients and key workers for infection with the causative SARS-CoV-2 virus. RT-PCR tests are highly…

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UncategorizedPosted onApril 1, 2020

Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays

Our findings characterize the limitations of commonly used primer-probe sets and can assist other laboratories in selecting appropriate assays for the detection of SARS-CoV-2. Our…

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UncategorizedPosted onMarch 31, 2020March 31, 2020

Antibody Responses to SARS-CoV-2 in Patients of Novel Coronavirus Disease 2019

Among 173 patients, the seroconversion rate for Ab, IgM and IgG was 93.1% (161/173), 82.7% (143/173) and 64.7% (112/173), respectively. Twelve patients who had not…

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UncategorizedPosted onMarch 22, 2020

Prolonged presence of SARS-CoV-2 viral RNA in faecal samples

Our data suggest the possibility of extended duration of viral shedding in faeces, for nearly 5 weeks after the patients’ respiratory samples tested negative for…

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UncategorizedPosted onMarch 21, 2020March 22, 2020

Virological assessment of hospitalized cases of coronavirus disease 2019

Preprint of a paper examining live viral loads and shedding in a cohort of patients in Germany. Link

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